EasyStain™ - IFA Reagent Against Cryptosporidium and Giardia

  • IFA Reagent Against Cryptosporidium and Giardia
  • 1gG1 Specificity
  • 15 Minute Incubation

EasyStain™ an immunofluorescence reagent designed for use in testing water samples for the presence of Cryptosporidium oocysts and Giardia cysts. When a water sample is stained with the EasyStain™ reagent all oocysts or cysts present in the sample will fluoresce bright green. Stained samples can then be examined using fluorescence microscopy, flow cytometry or laser scanning cytometry.

The detection of oocysts and cysts in water is extremely difficult. There are many particles present in water samples that cross-react with Cryptosporidium and Giardia-specific antibodies. These particles can easily be mistaken for Cryptosporidium oocysts or Giardia cysts. EasyStain™ does not cross-react with particles present in water samples. This superior specificity is achieved by using monoclonal antibodies (mAbs) of the IgGl isotype.

EasyStain™ utilises a patented method for producing IgGl antibodies to Cryptosporidium and Giardia. These antibodies have: been demonstrated to be far superior to other available antibodies.

The antibodies in EasyStain™ have been purified to a very high level to ensure that there is no contamination. The conjugated antibody is stored in a specially designed buffer that includes a novel blocking agent and preservative.

EasyStain™ is a mixture of two monoclonal antibodies {mAb): a Cryptosporidium-specific IgGl mAb and a Giardia-specific IgGl mAb. The reagent is supplied as a working solution and does not require dilution.

NEWS

BioBall™ Announcement
Further to the aquisition of BTF by bioMerieux in 2007, TCS Bosciences will no longer be distributing BioBall™

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Product Innovation

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EasyStain™ - an immunofluorescence reagent designed for use in testing water samples
Image courtesy: BTF©

Product Data

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EasyStain™

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A Wash Buffer is supplied that is used to wash excess antibody from slides or membranes. The Wash Buffer has beers specially designed to increase fluorescence and reduces fading. It does not require dilution.

A Positive Control, containing Cryptosporidium oocysts and Giardia cysts is supplied. Cysts and oocysts are at a concentration of 200 to 400 per 5O µl. Mounting Medium is supplied that is applied to the microscope slide before adding a coverslip. The Mounting Medium has been specially designed to reduce fading of the prepared sample.

EasyStain™ - Cryptosporidium and Giardia stanined with EasyStain IgG1

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TCS Biosciences Ltd. | Botolph Claydon, Buckingham, England, MK18 2LR

BSI Registered Firm - BS EN ISO 9001:2000, Certificate No.: FS 28907

Tel: +44 (0)1296 714222 ~ Fax: +44 (0)1296 714806 ~ E-mail: sales@tcsgroup.co.uk

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